C（3〜6か月） C (3-6 months) Recktm1(neo)Noda, global Reck knockout Recktm1(neo)Noda, global Reck knockout true In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 107, 789-800 (2001). 1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an<br>acknowledgment to the DEPOSITOR is requested.<br>2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.<br>3. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form.<br>4. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT shall reach agreement on terms and conditions of use of it with DEPOSITOR and shall obtain a prior written consent from the DEPOSITOR.<br>5. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Heterozygote x Wild-type [C57BL/6JJmsSlc] Homozygous mutant mice are embryonic lethal. Heterozygote x Wild-type [C57BL/6JJmsSlc] Homozygous mutant mice are embryonic lethal. 京都大学大学院医学研究科・高橋智聡先生、Regina M. Sasahara、Junseo Oh、理化学研究所脳科学総合研究センター・糸原重美先生ら。E14 ES細胞由来。 Reck遺伝子のノックアウトマウス。Reck遺伝子の第1エクソンをPGK-neoカセットで置換。ホモマウスは、血管新生、中枢神経発生などの障害を伴う胎生期致死となる。ヘテロマウスは、正常に発生し、交配可能であるが、一過性脳虚血後の脳障害の度合いや致死率が高くなる。, 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Cell, 107, 789-800 (2001). <br>1. 研究成果の公表にあたって謝辞の表明を必要とする。<br>2. 学術機関の学術研究に限る。<br>3. 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>4. 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。<br>5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Reck遺伝子のノックアウトマウス。Reck遺伝子の第1エクソンをPGK-neoカセットで置換。ホモマウスは、血管新生、中枢神経発生などの障害を伴う胎生期致死となる。ヘテロマウスは、正常に発生し、交配可能であるが、一過性脳虚血後の脳障害の度合いや致死率が高くなる。 B6.129P2-Reck<tm1Ito>/Noda B6.129P2-Reck<tm1Ito>/Noda E14 [129P2/OlaHsd] Developed by Chiaki Tkahashi, Regina M. Sasahara, Junseo Oh, Kyoto University Graduate School of Medicine, Shigeyoshi Itohara, RIKEN Brain Research Institute. E14 ES cells were used. The mutant mice were crossed to C57BL/6. Neurobiology Research RBRC05892 Reck gene knockout mice. Exon 1 of the Reck gene was replaced with a PGK-neo cassette. Homozygous mutant mice die around E10.5 with defects in collagen fibrils, basal lamina and vascular development. <a href='https://brc.riken.jp/mus/pcr05892'>Genotyping protocol -PCR-</a> 野田　亮 Makoto NODA Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Reck genomic DNA